Course Description: This course provides instruction on optimal protocols for the isolation, counting, freezing, thawing and quality control of peripheral blood mononuclear cells.

Course Format: This is a 30 minute self-paced course that includes slides, narration, four videos and two self-checks. A quiz at the end tests your knowledge and leads to a printable certificate if your score is 80% or higher. See additional criteria on the course page.

Viewing Requirements: You will need the Macromedia Flash Player 7.0 or higher to view this course. The player is a small file and downloads quickly. To go to the Macromedia web site and download the Flash player, click here.

Internet Connection: This course is best viewed with a high speed Internet connection.

Course Description:This course provides instruction on the flow cytometric analysis and enumeration of CD4+ T-cells for the monitoring of people infected with HIV. This program is intended as a training tool for laboratory technicians with basic knowledge of flow cytometry and as a refresher course for technicians with experience in flow cytometry.

Course Format: This is a 30 minute self-paced course that includes slides, narration, four videos and two self-checks. A quiz at the end tests your knowledge and leads to a printable certificate if your score is 80% or higher. See additional criteria on the course page.

Viewing Requirements: You will need the Macromedia Flash Player 7.0 or higher to view this course. The player is a small file and downloads quickly. To go to the Macromedia web site and download the Flash player, click here.

Internet Connection: This course is best viewed with a high speed Internet connection.

An interactive course taking approximately 30 minutes to compete. This course is intended as a training tool for laboratory technicians who process and manage specimens.

Topics include:

  • specimen collection
  • pickup
  • transport
  • processing
  • analysis
  • storage and disposal

This course was produced by Johns Hopkins Center for Clinical Global Health Education (CCGHE) and HIV Prevention Trials Network (HPTN) Virology Core Laboratory.


In this course you will learn how to:

  • Prepare and perform the HIV-1 Western Blot procedures
  • Observe the assay
  • Interpret the test results
  • Troubleshoot the results

Course Format: This is a 30 minute self-paced course that includes slides, narration, four videos and two self-checks. A quiz at the end tests your knowledge and leads to a printable certificate if your score is 80% or higher. See additional criteria on the course page.

This course was produced by Johns Hopkins Center for Clinical Global Health Education (CCGHE) and HIV Prevention Trials Network (HPTN) Virology Core Laboratory.